Effect of Purified Culture Filtrate of Rhizoctonia solani Isolate (UKMRSPL1) on the Detached Fresh Leaf of Parthenium

Parthenium control by using bioherbicide is highly demanded in Malaysia and elsewhere. A study was conducted to observe the effect of purified culture filtrate of Rhizoctonia solani (UMKRSPL1) isolate on the fresh detached leaf of parthenium at the laboratory of University Malaysia Kelantan, Jeli Campus. The isolated was cultured in Potato Dextrose Broth for 10 days. Filtrate was collected and was
extracted with methanol and without methanol. The filtrate was purified by treating with activated charcoal. The purified filtrate was centrifuged for 15 minutes and the
pallets were collected from the bottom of the centrifuged extracts. The purified and centrifuged extracts were applied on the detached leaf of parthenium at different concentrations of 0%, 2.2%, 5.0% and 10.0%. It was observed that 10.0% extract without methanol caused more 90% killing effect at day 8. The extract without methanol at 10.0% concentration caused only 55% killing effect in comparison to control.