The investigation on the analysis of air pollen samples was carried out by using a remote control helicopter in the atmosphere at six different locations in Pahang, Malaysia. Pollen in air is the main causal of allergic reactions. The objective of this research is to evaluate the number of pollen present in the air samples collected from different locations of Pahang, to identify the types of pollen based on pollen’s morphology in the air samples collected from different locations of Pahang and to identify the allergenic pollen present in the air samples collected. The chosen places were Kuantan, Bera, Jerantut, Temerloh, Raub and Kuala Lipis. Pollen count and pollen acetolysis method was carried out in order to determine the number of pollen loads in the atmosphere. A total number of 183 pollen grains were recorded in which consisted of 19 species from the six locations. Kuantan has the highest abundance of pollen loads (24.5 %) while Raub recorded the lowest abundance of pollen loads (8.2%) due to its climatic factors as well as geographical factors. Elaeis guineensis from Arecacea family was recorded as the most common pollen grains found in Pahang and it was previously identified as allergenic pollen.