Vegetable oil, often known as palm oil, is derived from the fruit of the oil palm tree, which is predominantly cultivated in tropical climates. It is a widely used oil in the culinary industry, as well as in the cosmetics and biofuels industries. There are 3 active constituents in oil palm: fatty acid, vitamin E and carotenoids. The aim of this research is to formulate the biosoap from palm oil and extract propolis from stingless beehive namely XbeeSoap. The two formulated samples from XbeeSoap different propolis extracts of 3 g and 6 g of propolis extracts were produced. Characterization of formulated XbeeSoap was carry out by investigating its functional group using FTIR, pH, moisture content and antimicrobial properties. The pH value of both sample XbeeSoap was 8. FTIR of the XbeeSoap indicated different functional group exist in the XbeeSoap like Aliphatic Hydrocarbons (C-H) and Primary Aliphatic Alcohol (O-H) which indicate the presence of propolis compound. While, the moisture content was resulted below 11% of the soap and the results of antimicrobial properties showed that sample 2 was the most efficient against all pathogenic organisms tested, with the maximum zone of inhibition (13 mm) against Bacillus subtilis and 12 mm against E.coli when administered at the highest concentration of 50 mg/mL. In conclusion, the development of this biosoap of XbeeSoap was successfully formulated and produced.