Serotyping of pasteurella multocida based on capsules in ducks in Kelantan, Malaysia

The present study was carried out on 65 duck liver to investigate the serotypes of Pasteurella multocida
causing fowl cholera in the state of Kelantan, Malaysia. These samples were obtained from different
farms in Kelantan which was preparing for marketing purpose during the period from August to
December 2013. Bacterial isolation, morphological identification, biochmical properties, and bacterial
DNA extration for PCR revealed that out of 65 samples 4 were P. multocida positive, confirmed by
PCR. The 4 samples identified produced species-specific 460bp band with KMT1T7 and KMT1SP6
primers.PCR serotyping using CAPA-FWD and CAPA-REV primers revealed that among 4 P.
multocida positive samples, 1 was type A strain having 1044 bp, and the other 3 strains were
untypeable. The present study revealed that P. multocida type A strain is prevalent in Kelantan.