Characterization and antibacterial activity of synthesized copper nanoparticles from Melastoma malabathricum leaves extract.

In the 21th century, the wide use of nanoparticles in different field had led to variety of methods of synthesis nanoparticles. Some method used had cause diverse impact in the environment. Thus, research on other method that more eco-friendly was required. In this study, the green synthesis approach was adopted to synthesize Copper nanoparticle (CuNPs) by using Melastoma malabathricum leaves extract. The prepared Copper nanoparticles were characterize by UV-visible spectroscopy (UV-vis), X-ray Powder Diffractometer (XRD), X-ray florescence (XRF) and Fourier Transform Infrared Spectroscopy (FT-IR). The UV-visible spectra of CuNPs revealed abroad absorption band between 370-380 nm contributing to the surface Plasmon vibration of metal nanoparticles. The XRF analysis confirmed the presence of silver in nanoparticles and XRD result reviled the monoclinique shape with 13-29 nm size of CuNPs. The presence of alcohol (O-H), alkene (C=C), organohalide (C-F) and oxygen ether (C-O) was confirmed by FT-IR analysis. Antibacterial activity of CuNPs against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration (0.003, 0.006, 0.009, 0.012, 0.015 and 0.02μg/ml) of nanoparticle enhance inhibition zone was observed.