Physical parameter and microbial screening in Lata Janggut, Jeli, Kelantan

Water microbe can be found widely in freshwater, ocean, lake or reservoir. Upstream
Lata Janggut, Jeli, Kelantan is chosen as the study area to culture and isolate the
microorganism from freshwater. Seven isolates microorganism were obtained. Based on
morphology characteristics which are shape margin and elevation, bacteria were
characterized. Result from Gram staining for the sample showed all the bacteria were
Gram positive bacteria. The shapes of bacteria obtained are coccus, dipplobacilli,
cocobacillus and bacillus. Seven isolates microorganism obtains undergo DNA
extraction to obtain DNA.16s rRNA universal primer was used to amplify the copy of
DNA by performing Polymerase Chain Reaction (PCR). The result from amplifying the
copy of DNA showed multiple bands. Optimizations of primer design can reduce
multiple bands. Number of control also should be considered when performing PCR.