Physical parameter and microbial screening in Lata Janggut, Jeli, Kelantan

Water microbe can be found widely in freshwater, ocean, lake or reservoir. Upstream Lata Janggut, Jeli, Kelantan is chosen as the study area to culture and isolate the microorganism from freshwater. Seven isolates microorganism were obtained. Based on morphology characteristics which are shape margin and elevation, bacteria were characterized. Result from Gram staining for the sample showed all the bacteria were Gram positive bacteria. The shapes of bacteria obtained are coccus, dipplobacilli, cocobacillus anbacillus. Seven isolates microorganism obtains undergo DNA extraction to obtain DNA.16s rRNA universal primer was used to amplify the copy of DNA by performing Polymerase Chain Reaction (PCR). The result from amplifying the copy of DNA showed multiple bands. Optimizations of primer design can reduce multiple bands. Number of control also should be considered when performing PCR.