The contamination of aquaculture waste in water in addition to anthropogenic activities has posed a great threat to ecosystem. The phytoremediation technology is used to treat the contaminated soil and water which containing higher amount of pollutants. In this study, Limnocharis flava has been used to remediate the pollutants contaminated water. The physico- chemical changes, removal efficiency of pollutants, accumulation of pollutants by Limnocharis flava was carried out in this research. All the samples were analysed by the XRF and Ysi 556 multi-parameter to identify the concentration of each pollutant in the water samples and physico-chemical parameter. The aquaculture wastewater and three reactor (Limnocharis flava) were put into reactor tank. Then, the pollutants concentration such as heavy metals and aquaculture waste based on hydraulic retention time (HRT) were analysed. The experiment is repeated with 6 cycles. The data analysis and statistics were calculated by Microsoft Excel 2013. The result of physico- chemical analysis showed that Limnocharis flava has the ability to increase the values of pH with 1.95 ± 4.48, DO with 12.85 ± 27.05 and reduce the value of TDS with -19.39 ± 66.30. Furthermore, the experiment demonstrated that Limnocharis flava significantly removed Ba with 30.88 ± 28.72 ppm, Sn with 51.7 2 ± 34.90 ppm, Al with 53.45 ± 49.78 ppm, P with 42.15 ± 19.64 ppm, S with 42.15 ± 26.38 ppm, Cl with 41.62 ± 25.88 ppm, and respectively in aquaculture wastewater. Moreover, Limnocharis flava can accumulate Al in plant tissues with BCF 0.0721 ppm. Therefore, Limnocharis flava have the characteristic that help to reduce pollutants from water sources and increase water quality.