Evaluation of insecticidal activity of a formulation of transfluthrin and cyfluthrin insecticide aerosol against lab-bred aedes aegypt/under exposed and unexposed position

A study on the evaluation of insecticidal activity of a formulation of in transfluthrin and cyfluthrin insecticide aerosol against lab-bred Aedes aegypti under open and hidden position was conducted. The formulation of aerosol used in this experiment designated as transfluthrin 0.040% w/w + cyfluthrin 0.025% w/w were commercially sold in the market as ahousehold insecticide product and was tested in a room furnished into similar bedroom condition. The formulation of aerosol was tested against Aedes aegypti based on the accordance of WHO guidelines for efficacy testing of household insecticides product (WHO/HTM/NTDPES/2009.3) and WHO guideline for safe and effective use of household insecticide product (WHOCDS/WHOPES/99.1). Four cages with 10 mosquitoes were placed in the following positions: one cage at the wall of room (approximately 2m up from the floor), one cage under the bed, one cage at the middle of the floor of the cabin room and another one cage at beside of the wardrobe. Ten lab-bred 3 years old sugar-fed Aedes aegypti adult mosquitoes were placed inside the test cages. The test were observed for knockdown at 5, 10, 15 and 20 minutes of exposure after applying the aerosol. The mortality rate was determined after 24 hours of holding period. The result showed that the transfluthrin 0.040% w/w + cyfluthrin 0.025% w/w are more effective among cages which placed in the open area rather than were kept in hidden places and also indicated that longer period of exposure time giving the highest knockdown effects