The present study focuses on Optimum Parameters (pH, Salinity, Formalin and Temperature) With Emphasis on The Hatching Rate of Climbing Perch, Anabas testudineus. This study was conducted in order to determine the best environment for climbing perch eggs to hatch and its resistance based on its hatching rate. The information from this study can help extensive, semi-intensive and intensive farmers to properly prepare the best condition for hatching climbing perch eggs. This study was carried out under laboratory condition where climbing perch eggs are placed in different temperature, pH, salinity concentration, and formalin concentration. The best condition for climbing perch eggs to hatch are 30°C, pH 5, 0 ppt of salinity and 0.01% of formalin. This finding shows that climbing perch eggs can tolerate slightly acidic, slightly high temperature, less salinity and formalin concentration. Nonetheless, there is need for further investigation in determining the best condition for climbing perch larvae to grow to adult size as this present study only covers early life cycle.