Antibacterial activity of Leucas Zeylanica leaves extract in elimination of bacteria

Leucas zeylanica, herbal medicinal plants belong to the Lamiacea family, genus Leucas are common being used widely in traditional medicinal to treat various diseases and bacterial infection. The antibacterial activity of most of the medicinal plant produced is commonly due to the constituents of active compounds. The aim of the study is to determine the antibacterial activity of L.zeylanica leaves extract in inhibition of gram positive and gram negative bacteria, E. coli and S. Aureus. The extraction of plant leaves is produced by soxhlet extraction and rotary evaporator. The solvent being used in this study is methanol. Different concentration of plant extract was used against two different bacteria strain. Antibacterial susceptibilities of L. zeylanica leaves extract were performed by using Disc-Diffusion method. The antibacterial activity was being assessed with diameter zone of inhibition on agar plate. In this study, the result then compared with standard antibiotic, Gentamycin as positive control and methanol solvent as negative control. The result obtained shows that 600 μg/μL concentration give maximum inhibition zone for both bacterial strain with range of (12.1±0.5mm) against E.coli and (16.3±0.9) against S.aureus. Active compound contain in medicinal plant that shows therapeutic potency in inhibit infection potentially believe able to further use in pharmaceutical field within future research.