Effect of TAPzyme 50a formulation on different fabrics in detergent spray application

This study involves protease from E. Coli BL21 (DE3) pLysS harbouring 50a protease gene, spray formulation detergent and its effectiveness performance analysis. Proteases are important enzymes that involved in many vital physiological processes and has wide potential for industrial applications. Four types of spray labelled namely distilled water, TAPzyme 50a, spray detergent and spray detergent without TAPzyme 50a. Stain removal bloodstains test were carried out on the different types of fabrics which are jersey, cotton, koshibo and crepe. This experiment had proved that with addition of TAPzyme 50a with the formulated detergent showed the most effectiveness to crepe fabric. During this study, the human bloods had used on different types of fabric to observe the most effectiveness by using different way of removal performance. Blood is a complete mixture of enzymes, proteins, cells and inorganic substances. It is suitable for this study that used TAPzyme 50a as a ingredient with combination of spray detergent. Bloostained on the fabrics were removed with distilled water, TAPzyme 50a, spray detergent and spray detergent without TAPzyme 50a. The combination of TAPzyme 50a with formulated detergent exhibited better removal bloodstains action by showing faintness of crepe fabric. The stains removal evaluation on different types of fabric with spray detergent obtained are as followed order: (Crepe > Jersey > Cotton > Koshibo), showed a great potential application of TAPzyme 50a in spray detergents application. The lightness of the fabrics after removal performance was measured by colorimeter.