Effect of TAPzyme 50a formulation to remove blood, egg yolk and milk stains on crepe cloth

This study involves protease production from recombinant bacterium, E. coli strain BL21 (DE3) pLysS harbouring 50a protease gene, formulation of liquid spray detergent and its dirt removal performance. Three types of stains (human blood, egg yolk and milk) have been stained on crepe cloth and its stain removal performance analysis with formulated liquid spray detergent through this study. Stain removal performance test were carried out. This experiment has proven that the combination of TAPzyme 50a with the formulated liquid spray detergent are the most compatibility and stability. It was exposed that through stain removal performance, the combination of TAPzyme 50a with formulated liquid spray detergent exhibited better stain removal action by showing of stains (human blood, egg yolk and milk). For blood stain, egg yolk and milk stains removal evaluation obtained as followed order: (formulated liquid spray detergent with TAPzyme 50a followed with TAPzyme 50 with distilled water, then formulated liquid spray detergent and lastly distilled water).