The abundant of bamboo waste has a potential as low cost adsorbent in waste water treatment. This study presents the potential of biochar from bamboo as adsorbent for removal of Reactive Orange 16 dye (RO16). There are several parameter have been studied such as effect of carbonization temperature, adsorbent dosage, initial concentration, contact time and pH. The results showed that the optimum value ware obtained at carbonization temperature of 800 ℃, adsorbent dosage of 2 g, intial concentration of 100 mg/L, contact time of 12 h and pH 6. The highest percentage for removal of RO16 dye using biochar bamboo (BB) was 99.94 %. The equilibrium data was best fitted on Langmuir adsorption isotherm with maximum adsorption capacity of 4.850 mg/g and R2 = 1.