Studies of combination of Azolla microphylla with silver nitrate and effect on Aedes aegpypti

Plant extract potential in synthesis the nanoparticles currently drawn attention in the field on nanotechnology. The development in nanotechnologies that contribute in many field had led to the demand of the nanoparticle production. Current nanotechnology for nanoparticle synthesis clarified toxic to the environment and costly. This impulse to find procedure that more environmentally friendly in synthesis the nanoparticles. In the view of the recently increased interest in biosynthesis of nanoparticles, this research studies about the combination of Azolla microphylla with silver nitrate with the purpose of synthesizing silver nanoparticles from Azolla microphylla extracts. This research also studying the optimal conditions in synthesis the nanoparticle and characterized their properties. In the present study, the synthesis of nanoparticle were performed by mixing the Azolla microphylla extract with I mM of silver nitrate solution. The colour changes into dark brown can be observed which indicate reduction process take place. The optimal conditions for synthesis silver nanoparticle were identified by testing volume ratio, time interval and pH parameter. Synthesized silver nanoparticles were then being characterized using UV-vis spectroscopy and FTIR. UV-vis spectrophotometer showed peak at 423nm due to plasmon surface resonance excited vibrations. FTIR analysis reveals the functional group presence in the silver nanoparticles. The research further with determine the effect of the combination of Azolla microphylla with silver nitrate on Aedes aegypti. The ability of other Azolla species in mosquitoes control in previous studies impulse the test on Aedes aegypti through 24-hour bioassay. The combination does not showed effective killing effect however repellent effect were proved. As the result the biological synthesis of nanoparticle were determined through the combination of the Azolla microphylla with silver nitrate in mosquitoes population might be give different effect due to different species.