Effect of salt concentration on enzyme-treated Black Soldier Fly Larvae (BSFL)

BSFL is an ideal protein source for animal feed as it maximize the profit and saving environment through prevent problem of wastage nutrient in the farm. However, the nutrients provided by BSFL are hard for animal to digest and absorb. Inorganic salt with high concentration had been reported has effect on protein denatured. They would destabilize the protein and followed by unfolded of protein which lead to protein denaturation occur. Denatured protein would become amino acid or polypeptides and helps in improve the absorption and digestion of protein. Three sample of salt being used in this study were sodium chloride, potassium chloride and sodium bicarbonate to examine the effect of salt on protease-treated Black Soldier Fly Larvae. 1, 5 and 10 weight per cent of three different salts was mixed with BSFL for 1 day in the room temperature before it 'treated with BSFL. The protein content was being determined by spectrophotometer. The result obtained showed the protein concentration decreases for all sample with the increasing of salt concentration. Protein concentration of control was 115.5 mg/mL and 11.78% of decreases in protein concentration. There were significantly differences for protein concentration for control and all samples. The percentage of decrease in protein concentration for sodium chloride, potassium chloride and sodium bicarbonate were higher in 10 weight per cent which were 25.49%, 25.26% and 30.11% in respectively. The order for the different types of salt to reduce the protein content of the BSFL were KCl > NaHCO3 > NaCI. This indicated that high concentration of salt would be more effective for reducing the crude protein of the BSFL. Treated BSFL could improve the animal's digestibility.