Antimicrobial activities of extract isolated from alpinia javanica (family zingiberaceae)

To find the efficiency of using herbs as part of alternative medicine against digestive bacteria involving Escherichia coli and Staphylococcus aureus, the extract of Alpinia javanica also known as “Lengkuas hutan” from different parts has been used. This monocotyledon plant belongs to the order Zingiberales where this plant consists of various compounds that potentially become antibiotic and have medicine properties. To test the efficiency and minimum Inhibition zone, the antimicrobial assay has been used throughout the Kirby-Bauer disk diffusion method by observing the inhibition zone of both bacteria that are present on the Petri dish. This assay involves three different parts of A. javanica involving leaf, pseudostem, and rhizome with different concentrations starting from 0 mg/ml, 2 mg/ml, 4 mg/ml, 6 mg/ml, 8 mg/ml, and 10 mg/ml. The minimum inhibitory concentration (MIC) has been determined after comparing the inhibition zone of three different parts of A. javanica at different concentrations. The finding shows that 2 mg/ml of extract from the leaf, pseudo stem, and rhizome was the minimum inhibitory concentration (MIC) as it shows the inhibition zone of both selected bacteria.