Analytical profiling of hawthorn fruit extracts using methanol and hexane solvent via gas chromatography-mass spectrometry (gc-ms)

An abstract of the research paper was presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement for the course DVT 55204 – Research Project. In this study, the Hawthorn fruit was extracted using both methanol and hexane solvents, followed by gas chromatography-mass spectrometry (GC-MS) to identify the chemical constituents of the hawthorn fruit. According to the GC-MS chromatography result for the methanol crude extraction of hawthorn fruit, five of the highest compounds were identified, with hexadecanoic acid and octadecyl ester (38.46%) showing the highest percentages. Then, followed by the n-Decanoic acid (20.05%), 5-Hydroxymethylfurfural (14.81%), beta.-D-Glucopyranose, 1,6-anhydro (2.27%), and lastly 9-Octadecenoic acid (Z)-, tetradecyl ester (2.19%). However, five different compounds were identified in the hexane crude extract from the same fruit. Tetracontane was found in the highest concentration (50.59%), followed by 7-Tetradecenal (11.65%), octacosyl trifluoroacetate (8.74%), n-Hexadecanoic acid (4.66%), and octacosane (3.21%). This result provides valuable insights for future research on isolating and applying these compounds for use in veterinary medicine as food additives or pharmaceutical preservatives.