Molecular detection of luxr homologue gene in vibrio sp.

The LuxR homologue gene plays a central role in quorum-sensing regulation among Vibrio species, influencing virulence, biofilm formation, and host–pathogen interactions. This study aimed to detect the presence of LuxR homologue genes in three Vibrio isolates obtained from aquatic environments using molecular techniques. Genomic DNA was extracted from each isolate, and PCR amplification was conducted using LuxR-specific primers targeting conserved regulatory regions. None of the three Vibrio isolates produced the expected amplicon corresponding to the LuxR homologue gene. The absence of LuxR-related sequences suggests that these isolates may rely on alternative quorum-sensing pathways, possess divergent LuxR-like regulators not amplified by the primers used, or belong to Vibrio species lacking conventional LuxR regulatory elements. These findings highlight the diversity of quorum-sensing mechanisms within Vibrio spp. and underscore the need for broader genomic screening or primer optimization in future studies.