This study studies the use of wood vinegar to promote spinach growth for ecologically friendly agriculture. Wood vinegar, a biomass pyrolysis byproduct, contains organic chemicals that boost plant development. The study evaluated plant development with wood vinegar, synthetic fertilizer, and no treatment over 26 days. Each treatment's soil pH was also assessed. Wood vinegar neutralizes soil pH at 6.4. Synthetic nutrient-treated soil had a pH of 5.2, whereas the untreated control group had 6.2. Plant area, height, and leaf count were compared to the untreated control to assess wood vinegar and synthetic nutrient benefits. These results illuminate how these treatments affect plant development and offer agricultural uses. Wood vinegar, synthetic nutrients, and no treatment were given to the control groups. To observe dynamic changes, plant growth was assessed on days 3, 7, 14, 18, 22, and 26. Data analysis shows huge trends in all indices. Growth was consistently better with wood vinegar than with synthetic nutrients or the untreated control group. On day 3, wood vinegar-treated plants had an area of 0.84 cm², a height of 2.4 cm, and 2 leaves. Plants treated with synthetic nutrients had a 0.72 cm² area, 2.1 cm height, and 2 leaves, whereas the untreated control group had a 0.77 cm² area, 2.2 cm height, and 2 leaves. The growth gap widened throughout the research. On day 26, wood vinegar-treated plants had a 71.07 cm² area, 14.6 cm height, and 8 leaves. Plants given synthetic fertilizers had a 65.0 cm² area, 13.8 cm height, and 7 leaves, whereas the untreated control group had 58.8 cm², 12.5 cm height, and 7 leaves. In sustainable agriculture, treatment selection may be based on their combined impact on plant physiology and soil conditions.