Determination of extraction method for β-glucan and evaluation wound healing ability of β-glucan from Proteus mirabilis PI18 strain

Microbial β-glucan have proved to be beneficial in various biomedical applications. In this work we explored the wound healing properties of β-glucan produced by newly isolated excessive β-glucan producing Proteus mirabilis PI18 strain. The β-glucan produced by P. mirabilis PI18 were extracted by three extraction methods and the chemical properties of the extracted β-glucan were characterized through Fourier transform infrared (FT-IR) spectroscopy. Would healing properties of the extracted β- glucan was tested on Sprague Dawley rats. The β -glucan was extracted and the analysis was followed by Fourier transform infrared (FT-IR) spectroscopy to characterize β (1→ 3) glucan extract from a newly isolated excessive beta-glucan producing Proteus mirabilis strain. All 20 Sprague Dawley rats were divided into 4 group: 1) Untreated, 2) Treated with commercial wound healing, 3) Treated with control β-glucan formulation, 4) Treated with β-glucan formulation. A full thickness skin excision was performed to all rats. Treatment of the rats were given for 21 days. The effect of β-glucan produced by P. mirabilis PI18 strain on wound morphology and histopathological findings of wound recovery are evaluated on Sprague-Dawley rats. In this work, the β-glucan formulation was effective in terms of its ability to promote wound healing on animals and can further developed for wound healing treatments.