Fusarium solani and Aspergillus niger are ubiquitous filamentous fungi found worldwide in diverse environments such as soil, decaying organic matter, and indoor settings. While their exact origins are not definitively known, both species have cosmopolitan distributions, reflecting their adaptability to various habitats. Fusarium solani is notorious for its role as a plant pathogen, causing diseases such as root rots and wilts in agricultural crops. Similarly, Aspergillus niger is versatile, participating in organic matter decomposition, but can also pose problems as a contaminant in food processing and indoor environments, potentially affecting human health. One of its benefits for culture is gaharu. It is because both fungi are the main infector for resin production in gaharu agarwood. Purpose of this study is to determine the shelf life of Fusarium solani and Aspergillus niger on different parameter. Light microscopy analysis of the fungi to determine the morphology of desired fungi, while Optical Density use for determination of availability of the cell. Overall, this study provides insights into the effectiveness of storage time and temperature for availability of Fusarium solani and Aspergillus niger.