Investigation of indole-3-acetic acid production and bioremediation activity of Pseudarthrobacter sp UMK-PNF5

Agriculture is vital for global food production and environmental health, which are interconnected aspects crucial for human well-being and ecosystem vitality. Pseudarthrobacter sp. and the importance of IAA (indole-3-acetic acid) are closely intertwined in the context of plant-microbe interactions. IAA, as a plant hormone, plays a crucial role in various physiological processes of plants, including cell division, elongation, and differentiation. Pseudarthrobacter sp., being capable of producing IAA, can potentially influence plant growth and development positively. Through the secretion of IAA, Pseudarthrobacter sp. can enhance root growth, improve nutrient uptake efficiency, and confer resistance to biotic and abiotic stresses in plants. Consequently, this bacterium holds promise as a plant growth-promoting bacterium (PGPB) and a valuable component of sustainable agricultural practices aimed at enhancing crop productivity and resilience. However, current production levels of Indole-3-acetic acid (IAA) by Pseudarthrobacter sp. fall short of practical use. Conventional methods for addressing environmental challenges from industrial activities are both expensive and generate toxic byproducts. In contrast, bioremediation is an eco-friendly technique that shows promise for effectively eliminating pollutants. Pseudarthrobacter sp. has demonstrated its ability to degrade various toxic substances, making it an ideal candidate for evaluating the remediation potential of Pseudarthrobacter sp. UMK-PNF5. This study aims to investigate the production of IAA by Pseudarthrobacter sp. UMK-PNF5 under different concentrations of L-tryptophan, specifically 1000, 3000, 6000, and 9000 μg/mL. Additionally, the study examines the IAA production of Pseudarthrobacter sp. UMK-PNF5 using different carbon and nitrogen sources, including molasses and fructose as carbon sources, and beef extract and tryptone as nitrogen sources, with the addition of 1000 μg/mL concentration of L-tryptophan. It shows higher absorbance produces higher IAA. Furthermore, the research explores the bioremediation capacity of Pseudarthrobacter sp. UMK-PNF5 in breaking down phenol, providing insights into optimal growth conditions and residual phenol determination. These findings show Pseudarthrobacter sp UMK-PNF5 cannot degrade phenol. These findings enhance our understanding of the capabilities of Pseudarthrobacter sp. UMK-PNF5 offers potential applications in both sustainable agriculture and environmental remediation.