The study addresses the challenge of optimizing biodiesel production methods, considering the time, cost, and resource-intensive nature of conventional testing techniques. The deficiency in a cost-effective and comprehensive approach prompts the exploration of Aspen Plus® simulation as a tool to enhance biodiesel production techniques. The objective is to develop an online environment for researching and optimizing the process parameters, catalysts, and operational conditions, with the aim of improving efficiency, reducing expenses, and expediting the discovery of environmentally friendly and profitable biodiesel production approaches. The research employs Aspen Plus® simulation software to simulate biodiesel production from waste cooking oil using various heterogenous catalysts such as crab shell and plantain peel, tungsten and activated charcoal. The efficiency of these heterogeneous catalysts is evaluated by comparing biodiesel yield and purity. Additionally, the performance of bifunctional heterogeneous catalysts is compared with other catalysts commonly used in biodiesel production. The methodology involves Aspen Plus® simulation and transesterification as the primary process, utilizing waste cooking oil, methanol, and catalysts. The viability of the suggested base case study is assessed through process simulation, emphasizing glycerol management and optimizing transesterification conditions for the production of 80000 tonnes per year of biodiesel. The outcomes of this study proven that heterogenous bifunctional catalyst has produced highest yield and purity compared to others. They also contribute to a more sustainable and economically viable biodiesel production process.