This research aims to develop and analyze the properties of mixed matrix ultrafiltration membranes combined with aluminum oxide nanoparticles for the purpose of humic acid removal. This membrane was developed by integrating polyethylene sulfone (PES) as the main polymer, with the addition of aluminum oxide nanoparticles (Al2O3NPs) as a biopolymer. The main objective of this ultrafiltration membrane is to improve the separation efficiency and resistance to organic pollution in humic acid. The membrane manufacturing process involves the preparation of a PES solution that has been doped with a predetermined weight percentage of PES and Al2O3NPs. Several membrane compositions have been developed to investigate the effect of varying Al2O3NPs content on membrane performance. The optimal thickness of the flat membrane is achieved through the dry phase drying method. The physical and chemical properties of the membrane have been thoroughly studied. Membrane hydrophobicity was determined through contact angle measurements, while hydrodynamic parameters such as separation ability and membrane filtration ability were evaluated. Fourier Transform Infrared Spectroscopy (FTIR) analysis was carried out to explore the chemical structure of the membrane and the interaction between PES and Al2O3NPs. The conclusion of this study is expected to provide a comprehensive understanding of the potential application of mixed matrix ultrafiltration membranes with Al2O3NPsin improving separation efficiency and membrane resistance to water pollution. Implications of this technology for water pollution treatment, especially for humic acid treatment.