In this work, the characterization of Agar hydrogel electrolytes of zinc-air batteries was studied. Issues with synthetic polymer hydrogels include a lack of inherent bioactivity while having acceptable mechanical and other properties. Hydrogel electrolytes are ecologically safe, biocompatible, degradable, and have inherent flexibility, which makes them ideal for real-world energy storage applications. Agar powder was first prepared by dissolving it with 100 mL distilled water and heated until 80 – 100 ̊C to form hydrogels. The ideal Agar hydrogel composition was 1:10, and it was dissolved in various KOH concentrations to create an electrolyte. This study's characterization was Open circuit potential (OCP), FTIR, morphological, and XRD. A 4M KOH was recorded for the best conductivity in the agar hydrogel electrolyte.