Constant Flow Rate Feeding Fed-Batch Fermentation Kineti Modelling of Docosahexaenoic Acid (DHA) Production by Schizochytrium sp.

DHA production fiom fementation process is one of the alternative to substitute DHA production fom fish oil which may have some harmful effects due to contaminant.
Soltooliveam sp. is one of the microorganism species that can be used for production of DHA but the fermentation process conducted towards this species is quite scarce. The bish cost in fermentation optimization study is one of the concern in the production of DHA. Therefore the study focus on developing and validating fed-batch kinetic model as an approach to reduce the cost needed for optimization study. Sensitivity analysis was abo done to measure the significant of the specific parameter. In this study, model development, parameter estimation and sensitivity analysis was done to achieve the objectives of the study. The results show that Logistic model, substrate consumption model and Leudiking-Piret model fit with the experimental data and valid to use based on the high coefficient determination value. The sensitivity analysis show that all three model are valid when simulated with different flowrate but only substrate utilization rate is vald when simulated with different feed concentration. Therefore, the model proposed can be used to represent the fermentation process. This study will help the researcher to predict the possible result by using the parameter desired. Therefore they can choose the significant factor that effect the fermentation process and reduce cost during DHA optimization study in Schizochytrium sp.