Phytochemical Screening And Antioxidant Ivity Of Crude Plant Extracts From Kyllinga Brevifolia (Green Kyllinga) Plant

Kyllinga Brevifolia is believed to have great potential as herbal medicine for humans and animals due to the presence of phytochemical compounds such as alkaloids, flavonoids, terpenoids, and others. In this study, maceration was used to extract the phytochemical components from the plant with two different solvents, which are distilled water and ethanol. The determination of the phytochemical constituent in the plant and several screening tests have been conducted, including Salkowski's test, Dragendorffs test, Alkaline reagent test, and some other chemical tests. The effect of solvents was observed towards the presence of bioactive compounds. The analysis of the antioxidant activity in the plant was done using DPPH free radical scavenging activity assay method. Phytochemical screening results showed the presence of the expected bioactive compound in the plant extract. However, tannins and steroidal glycoside are absent in distilled water extract, while saponins and flavonoids presented negative results in ethanol extracts. Next, the antioxidant activity in plant extract is higher than ascorbic acid based on the results of DPPH radical inhibition and ICso. The value of ICso for distilled water extract is 6.973 mg/mL that considered as strong antioxidant activity followed by ethanol extract (25.098 mg/mL) and ascorbic acid (37.409 mg/mL). Compared with ethanol extract, distilled water is more suitable for studying the antioxidant activity in Kyllinga Brevifolia with the lowest ICso value.
Keywords: Kyllinga Brevifolia, Phytochemical compounds, Effects of solvents,
Antioxidant activity