Giant freshwater prawn or scientifically known as Macrobrachium rosenbergii production well known for years due to its prominent size and good flesh quality. Although giant freshwater prawns’ economic sector is popular among all sort of people, there’s still problem risen from this production sector. The high-cost part in aquaculture production is the feed cost, comprises about 70% of total production cost. This has limits expansion of aquaculture industry especially for a small and medium scale farmer. In most studies, BSFL has proved to successfully replace commercial animal feed which BSFL has similar amino acid (AA) profile as fish meal (FM). Application of BSFL introduced as an alternative feed for M. rosenbergii might be potential. BSFL with other ingredients like rice bran, fish meal, corn meal, soybean meal and copra meal was mixed using feed formulation that was determined in Win feed software version 2.8. Different percentage of BSFL which are at 10%, 20%, 30% and 40% were used in feed formulation while formulation without BSFL will be used as control. Biochemical composition of all treatments were determined using proximate analysis on crude protein, crude fat, crude fibre, ash and moisture. For crude protein, treatment 4 (40%), (30.82 ±0.19) has the highest percentage compared to other treatments, Control, Treatment 1 (10% BSFL), Treatment 2 (20% BSFL) and Treatment 3 (30% BSFL), (23.01 ±0.13, 25.63 ±0.05, 28.29 ±0.5, 30.01 ±0.08). For crude fat, the highest percentage of crude fat is (12.77 ±0.38) for treatment 4 compared to other treatments , Control, Treatment 1 (10% BSFL), Treatment 2 (20% BSFL) and Treatment 3 (30% BSFL), (5.00 ±0.97, 6.08 ±0.66, 9.29 ±0.34, 12.52 ±1.22). For crude fibre, the highest treatment is treatment 2 (11.67 ±0.76) while for ash, the highest percentage is control ( 0.25 ±0.05). The treatment with high percentage of moisture is Treatment 4 with (26.66 ±0.37). The result shows that formulation with 40% of BSFL fulfil the nutrients requirement for M. rosenbergii juvenile. The other formulation did not fulfil the nutrients requirement in term of protein, fat, and fibre.