Antibiogram of Vibrio parahaemolyticus isolated from diseased Climbing perch (Anabas Testudineus) of aquarium shop

The ornamental fish industry has contributed to billions of dollars market value in the world. However, Vibrio parahaemolyticus is posed a threat to the industry where it might cause a severe economic loss but there is scarce information available related to the V. parahaemolyticus infection in ornamental fish. In the present study, V. parahaemolyticus was isolated and identified from a diseased Anabas testudineus from local aquarium shop. Thiosulphate-citrate-bile salts-sucrose (TCBS) agar was used to isolate V. parahaemolyticus and BBL Crystal kit was used for identification. A total of 30 bacteria colonies was isolated from abdomen, eye, gill, kidney and skin were tested to a total of 16 types of antimicrobial susceptibility test discs by using disk diffusion method. The antimicrobial susceptibility test tested in this study were amoxicillin (25 μg), ampicillin (10 μg) chloramphenicol (30 μg), compound sulphonamides (300 μg), doxycycline (30 μg), erythromycin (15 μg), flumequine (30 μg), fosfomycin (50 μg), kanamycin (30 μg), nalidixic acid (30 μg), novobiocin (30 μg), oxolinic acid (2 μg), oxytetracycline (30 μg), spiramycin (100 μg), sulphamethoxazole (25 μg) and tetracycline (30 μg). It was found that all isolates have higher resistance rate towards compound sulphonamides (96.6%), ampicillin (76.6%), sulphamethoxazole (70%) and amoxicillin (66.6%), but exhibit sensitivity to oxolonic acid (76.6%). The antimicrobial susceptibility test indicated that oxolonic acid is the one that effective in treat V. parahaemolyticus infections efficiently. The multiple antibiotic resistance (MAR) index for V. parahaemolyticus was 0.81. Thus, it indicates that the samples were highly exposed to the test antimicrobials.