The most important method of controlling insects, mites, weeds, and plant disease is biological control. The natural enemy of insect pests, also known as a biological control agent, includes predators like Oecophylla smaragdina. The 0. smaragdina is the most effective natural enemy for controlling pest insect species. Because it feeds and controls a large number of pests, including eggs, larvae, and adults of various insect species, 0. smaragdina is commonly used as a bio-controller in fruit orchards around the world. In this study, the effectiveness of 0. smaragdina versus wood vinegar in controlling_ pests was compared. The research was carried out in a 20-year-old Citrus suhuiensis cv. limau madu orchard in Ayer Lanas. With the total of 24 trees, a completely randomised design was used. The trees in this orchard are divided into three treatments, with one treatment applied to each of the eight trees. Ti is the tree that was treated with O.smaragdina, T2 is the tree that was treated with wood vinegar, and T3 is the tree that was not treated with ants and wood vinegar. To trap the pest, yellow stickers wer placed on all of the trees. The number of pests was significantly different depending o the type of treatment (p<0.05), indicating that O.smaragdina was effective in pes control. Because the beneficial insect suppresses the population of fruit flies, the resu of fruit fly infestation on the fruit does not differ significantly (p>0.05) betwee O.smaragdina and wood vinegar.