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Phytochemical study and DNA cleavage activity of citrus maxima ethanolic fruit extract


Citation

Zakia Khanam and Nur Hazerra Mohd Zakaria and Kong, Hui Sam and Chua, Hui Ching and Muhammad Che Isa and Irshad Ul Haq Bhat Phytochemical study and DNA cleavage activity of citrus maxima ethanolic fruit extract. In: Proceedings of 4th USM-PSU-NSTRU-MU International Conference on Arts and Sciences, 2015, Universiti Sains Malaysia. (Submitted)

Abstract

Citrus maxima Merr is a tropical fruit from rutaceae family. Fruits of the plant had been utilized in folk
medicine and showed various pharmacological properties. The present study include phytochemical screening, quantitation and identification of phenolics in fruit ethanolic extract of C. maxima. Furthermore, C. maxima ethanolic extract was evaluated against pathogenic E.coli palmid DNA by DNA cleavage assay. Phytochemical screening was carried out by colour reaction. The total phenolic (TPC) and total flavonoid content (TFC) were determined by Folin-Ciocalteu and aluminium trichloride colorimetric method, respectively. The identification of C. maxima phenolics present in ethanolic extract was done by HPLC equipped with Diode Array Detector. DNA cleavage activity was performed by gel electrophoresis method at various concentrations of the extract (0.125 pg/ul to 0.600 pg/ul). The preliminary phytochemical analyses, TPC and TFC results indicated C. maxima ethanolic extract is a
good source of phenolics. 4-hydroxy-3-methoxy cinnamic acid and quercetin were identified as major phenolic acid and flavonoids in the ethanolic extract respectively. DNA cleavage activity exhibited ability of maximum bacterial DNA cleavage up to the linear form in a concentration dependent manner. Thus, overall results of the study suggests, C. maxima possess neutraceutical potential and may be exploited in future for therapeutic applications and anticancer drug discovey.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Collection Type: Institution
Uncontrolled Keywords: phytochemical screening; phenolic acid; flavonoid; HPLC; DNA cleavage
Faculty/Centre/Office: Faculty of Agro - Based Industry
Faculty of Earth Sciences
Depositing User: Repository Admin
Date Deposited: 16 Nov 2016 07:51
Last Modified: 23 May 2022 11:17
URI: http://discol.umk.edu.my/id/eprint/9182
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