Digital Special Collection Portal

An efficient in vitro regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.-A dye yielding plant


Citation

Mohammed, A. and Chiruvella, K.K. and Namsa, N.D. and Ghanta, R.G. (2015) An efficient in vitro regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.-A dye yielding plant. Physiology and Molecular Biology of Plants, 21. pp. 417-424.

Abstract

Bixa orellana L. (Bixaceae) is a multipurpose tree grown for the production of commercially important dyes. In the present study, an efficient, reproducible protocol was developed for direct plant regeneration from in vitro derived petiole explants of Bixa orellana L. Murashige and Skoog medium (MS) supplemented with 2-isopentenyl adenine (9.8 μM) and naphthalene acetic acid (10.7 μM) was found to be optimum for production of high frequency of shoot organogenesis. Subculturing of the shoots onto the fresh MS medium containing similar concentrations of 2-iP (9.8 μM) and NAA (10.7 μM) produced elongated shoots. Elongated shoots when placed onto MS medium supplemented with 1.7 μM indole-3-acetic acid and 14.7 μM 2-iP produced optimal rooting. Rooted plantlets were acclimatized and transplanted to the field successfully. Histological investigation revealed the origin of shoot primordia, from sub-epidermal cells of petiole explants. The regeneration protocol developed in this study can be useful for mass in vitro propagation and effective genetic transformation of commercially important edible dye yielding tree species

Download File / URL

Full text not available from this repository.

Additional Metadata

Item Type: Non-Indexed Article
Collection Type: Institution
Date: 2015
Journal or Publication Title: Physiology and Molecular Biology of Plants
Uncontrolled Keywords: Achiote; Adventitious roots; Histology, rooting, petiole; Lipstick tree
Faculty/Centre/Office: Faculty of Agro - Based Industry
URI: http://discol.umk.edu.my/id/eprint/8157
Statistic Details: View Download Statistic

Edit Record (Admin Only)

View Item View Item

The Office of Library and Knowledge Management, Universiti Malaysia Kelantan, 16300 Bachok, Kelantan.
Digital Special Collection (UMK Repository) supports OAI 2.0 with a base URL of http://discol.umk.edu.my/cgi/oai2