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Non-protein coding rna genes as the novel diagnostic markers for the discrimination of salmonella species using pcr


Citation

Ravichantar Nithya and Siti Aminah Ahmed and Chee-Hock Hoe and Subash C. B. Gopinath and Marimuthu Citartan and Suresh V. Chinni and Li Pin Lee and Timofey S. Rozhdestvensky and Thean-Hock Tang (2015) Non-protein coding rna genes as the novel diagnostic markers for the discrimination of salmonella species using pcr. Plos One Journal, 10 (3). pp. 1-16. ISSN 1932-6203

Abstract

Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S. Typhi non-protein coding RNA (sRNA) genes. With the designed sequences that specifically detect sRNA genes from S. Typhi and S. Paratyphi A, a detection limit of up to 10 pg was achieved. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, we have attained a respective detection limit of 15 and 1.5 CFU/mL. The designed strategy using sRNA genes shown here is comparatively sensitive and specific, suitable for clinical diagnosis and disease surveillance, and sRNAs represent an excellent molecular target for infectious disease.

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Additional Metadata

Item Type: Non-Indexed Article
Collection Type: Institution
Date: 2015
Journal or Publication Title: Plos One Journal
ISSN: 1932-6203
Uncontrolled Keywords: Non-protein coding rna - Salmonella - Salmonellosis
Faculty/Centre/Office: Faculty of Veterinary Medicine
URI: http://discol.umk.edu.my/id/eprint/7994
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