Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast

This study was carried out to compare the surface
morphological changes and cell-to-cell attachment
with different concentrations of gentamicin-coated
hydroxyapatite (HA) on the osteoblast cell lines.
Osteoblast cell lines were cultured and maintained in
complete medium, 1:1 HAM’s F12 Medium
Dulbecco's modified Eagle's medium without phenol
red (DMEM) and incubated at 37oC in 5% CO2. The
cell lines were treated with gentamicin-coated HA
and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5-
diphenyl tetrazolium bromide (MTT) assay. After 72
hours treatment, the gentamicin-coated HA was
processed for viewing the morphological changes by
using scanning electron microscopy. The MTT assay
results indicated that varying concentrations of
gentamicin coated hydroxyapatite do not significantly
affect viability of osteoblast. The osteoblast-like cells
attached and spread well on the surface of
hydroxyapatite and grew into the pores. In
conclusion, the gentamicin-coated HA is
biocompatible towards osteoblast.