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Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper


Lee Chee Keong and Darah Ibrahim and Ibrahim Che Omar (2013) Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper. Malaysian Journal of Microbiology, 9 (1). pp. 84-92. ISSN 2231-7538


Aims: It is recognized that laser printed paper are difficult to deink using conventional method. This had lead to the
suggestion of enzymatic approach to overcome the problem encountered by commonly employed deinking techniques.
The present study aimed to investigate 7 commercially available enzymes for their suitability use in deinking of laser
printed paper.
Methodology and results: 3 cellulases, hemicellulases, xylanase and 2 lipases were used in enzymatic deinking of
laser-printed wastepaper. Cellulase A “Amano”3 (C), Hemicellulase (H) and lipase (L) were selected for used in deinking
because they possess either highest activity or broad pH stability compared to others enzymes. Different combination of
enzymes was carried out to evaluate their effectiveness in deinking process. CH enzymes sequence was determined to
be the most effective sequence in toner removal with 1.90% of brightness increment. However, only 0.95% of brightness
increment was gained by enzyme sequence L. Highest deinking efficiency obtained was not proportional to the highest
total reducing sugar produced.
Conclusion, significance and impact of study: Enzyme (cellulase and hemicellulase) can be used to de-ink laserprinted
wastepaper, which are difficult to be deinked by conventional chemical deinking process. Thus, enzyme deinking
has high possibility as alternative method to current chemical deinking process which is not environmental friendly.

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Additional Metadata

Item Type: Indexed Article
Collection Type: Institution
Date: 2013
Journal or Publication Title: Malaysian Journal of Microbiology
ISSN: 2231-7538
Uncontrolled Keywords: Laser-printed paper - Cellulase - Hemicellulase - Lipases - Enzymatic deinking
Subject Heading: Hemicellulose
Subject Heading: Cellulose
Faculty/Centre/Office: Faculty of Agro - Based Industry
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