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Differential expression of the vacuolar aspartic proteinase (APR1) gene in Candida albicans strains isolated from Malaysian and Iranian human and mice blood


Citation

Erkihun Aklilu and Amri Saroukolaei S and Shokri H (2012) Differential expression of the vacuolar aspartic proteinase (APR1) gene in Candida albicans strains isolated from Malaysian and Iranian human and mice blood. African Journal of Microbiology Research, 6 (9). pp. 2150-2155. ISSN 1996-0808

Abstract

Intracellular proteinase A is an aspartic enzyme in Candida albicans that is expressed by APR1 gene. The aims of this study were to evaluate and to compare APR1 gene expression in C. albicans strains isolated from Malaysian and Iranian patients and mice infected by C. albicans obtained from Malaysian and Iranian patients. The evaluation of APR1 gene expression was performed using reverse transcriptase-polymerase chain reaction (RT-PCR) with two-pair primers and 18S rRNA as housekeeping gene. The expression of APR1 gene and 18S rRNA was determined on agarose gel electrophoresis. The results did not show any significant difference in APR1 gene expression between C. albicans isolated from mice blood infected by C. albicans isolated from Malaysian and Iranian patients (p > 0.05). The expression of APR1 gene in C. albicans strains isolated from Malaysian patients was more than Iranian patients (p < 0.05). In addition, the gene expression in C. albicans strains obtained from mice blood was significantly higher than C. albicans strains isolated from both Malaysian and Iranian patients (p < 0.05). Considering the important role of intracellular proteinase A in C. albicans, APR1 gene as producer of this enzyme can act as an important gene in virulence of C. albicans in different environmental situations.

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Additional Metadata

Item Type: Indexed Article
Collection Type: Institution
Date: 9 March 2012
Journal or Publication Title: African Journal of Microbiology Research
ISSN: 1996-0808
Uncontrolled Keywords: Candida albicans - APR1 gene - Reverse transcriptase -Polymerase chain reaction (RT-PCR) method.
Subject Heading: Candida albicans
Subject Heading: Reverse transcriptase
Subject Heading: Polymerase chain reaction
Faculty/Centre/Office: Faculty of Veterinary Medicine
URI: http://discol.umk.edu.my/id/eprint/7456
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