Potential of antibacterial and antioxidant properties on papaya leaves crude extract

Papaya is one of the medicinally valuable plants belonging to the family Caricaceae. Traditionally, papaya leaves are used in the treatment of diseases. The papaya
leaves were collected from Bukit Mertajam, Pulau Pinang, grinded and sequentially extracted with hexane, ethanol, acetone, ethyl acetate and distilled water by using Soxhlet extraction method. The crude extract was subjected for acid value test. The lowest acid value was obtained by acetone extract of papaya leaves in both powder and dried leaves formed, ethanol extract of papaya leaves in powder formed, ethyl acetate extract of papaya leaves in dried leaves formed and distilled water extract of papaya leaves in powder formed. The antibacterial potential of the crude extracts on E.coIi and Staphylococcus aureus was examined using disc diffusion method. It was observed that all extraction conditions showed activity against bacteria except for distilled water extraction of crude extract of papaya leaves in powder and dried leaves formed. The antioxidant potential was evaluated by 2, 2-diphenyl-picrylhydrazyl assay. It was observed that all extraction conditions showed antioxidant property. In the present study extraction of papaya leaves has been analyzed using GC-MS. Eighteen chemical elements has been diagnosed and the active component identified was oleic acid.