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Antioxidant studies of the banana leaves (Musa acuminata)


Nur Rasyidah Muhammad (2016) Antioxidant studies of the banana leaves (Musa acuminata). Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)


Musa acuminata compound was successfully isolated and screened for their antioxidant activity. Musa acuminata leaves were macerated by using four types of different solvents which are methanol, acetone, hexane and distilled water. Thin layer chromatography (TLC) and column chromatography were used to isolate the crude
extracts and fractions by using different type of solvent system. The antioxidant activity of banana leaves were evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic content (TPC) assay and total flavonoids content (TFC) assay. Methanol crude extract was selected as best crude extract. The best solvent to separate methanol extract was ethyl acetate. Ethyl acetate was also the best solvent for the first and second chromatography fraction. HPLC result analysis confirmed the selected fraction has single compound. Methanol extract and selected fraction shows low activity toward the DPPH assay but high antioxidant activity in total phenolic and total flavonoids content.

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Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2016
Call Number: SBT 2016 047
Supervisor: Dr. Krishna Veni A/P Veloo
Programme: Bioindustrial Technology
Institution: Faculty of Agro - Based Industry
Faculty/Centre/Office: Faculty of Agro - Based Industry
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