Digital Special Collection Portal

Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants


Nor Mazalfala Aridzi (2016) Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)


Introduction : Microbial metal reduction forms the basis of alternate bioremediationstrategies for reductive precipitation and immobilization of toxic metals such as Fe(III). Objectives : A rapid screening techniques were developed to identify Shewanella oneidensis MR-1 respiratory mutants unable to reduce Fe(III) as anaerobic electron acceptor. Method : The Fe(III) reduction-deficient mutant screening technique was based on the observation that wild-type Shewanella Oneidensis produced a dark blue precipitate on its colony surface during growth on Fe(III) while colony arising from
mutagenesis did not.

Result : Three of the mutant strains were unable to produce the dark blue precipitate
after tested for anaerobic growth in a 96-well plates. The result present study shows that Shewanella Oneidensis iron-reduction deficient mutants was generated and yet
retained the ability of reducing Fe(III) to Fe(II).

Conclusion : This study describes the development of a mutant screening technique and its application to identify a set of mutants in a metal-reducing member of the genus Shewanella in anaerobic condition which is use in a bioremediation process.

Download File / URL

Full text not available from this repository.

Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2016
Call Number: SBT 2016 044
Supervisor: Dr. Wee Seng Kew
Programme: Bioindustrial Technology
Institution: Faculty of Agro - Based Industry
Faculty/Centre/Office: Faculty of Agro - Based Industry
Statistic Details: View Download Statistic

Edit Record (Admin Only)

View Item View Item

The Office of Library and Knowledge Management, Universiti Malaysia Kelantan, 16300 Bachok, Kelantan.
Digital Special Collection (UMK Repository) supports OAI 2.0 with a base URL of