Digital Special Collection Portal

Optimisation of random amplified polymorphic DNA (RAPD) protocol for identification of molecular markers in selected wild banana species


Citation

Muhammad Luqman Hasbullah (2016) Optimisation of random amplified polymorphic DNA (RAPD) protocol for identification of molecular markers in selected wild banana species. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)

Abstract

Banana (Musa spp) is one of important staple food in the world. But, the disease infected to banana species make the species vulnerable to disease. It is suggested to
plant resistant cultivars rather than using chemical control. The source of resistance plant may be find from wild species. To find the trait of gene must be assist by
molecular marker such as RAPD, AFLP or ISSR. But then, all the assistance of molecular marker need to be optimise to get the best bands. In this study, molecular
techniques specifically RAPD were used to analyse genetic traits. Optimisation of annealing temperature and concentration of MgCl₂ were the focus. Selection of primers also one of the main factors to determine the succesful results. Furthermore, the use of appropriate primer combination could enable the development of DNA
fingerprint for genetic diversity in producing of monomorphic and polymorphic bands. In conclusion, it is suggested to use primer OPJ-04, OPH-09, OPA-06, OPA-19 and OPJ-13 to get the best combination of polymorphic and monomorphic. This research also suggest to use 36.6°C for annealing temperature and 3.0 mM of MgCl₂ concentration as they are the optimum annealing temperature and concentration for all the primers tested.

Download File / URL

Full text not available from this repository.

Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2016
Call Number: SBT 2016 036
Supervisor: Dr. Fatimah Binti Changgrok @ Kayat
Programme: Bioindustrial Technology
Institution: Faculty of Agro - Based Industry
Faculty/Centre/Office: Faculty of Agro - Based Industry
URI: http://discol.umk.edu.my/id/eprint/6565
Statistic Details: View Download Statistic

Edit Record (Admin Only)

View Item View Item

The Office of Library and Knowledge Management, Universiti Malaysia Kelantan, 16300 Bachok, Kelantan.
Digital Special Collection (UMK Repository) supports OAI 2.0 with a base URL of http://discol.umk.edu.my/cgi/oai2