Antioxidants activity in different parts eleiodoxa conferta fruit

Most indigenous fruits of Malaysia are still underutilized and Eleiodoxa conferta is one of the examples. Currently, there are raising interest to replace synthetic antioxidants with natural antioxidants. Underutilised fruit can be a good source of antioxidants. The antioxidant activity of the fruit was evaluated by measuring the total phenolic content and percentage of free radical scavenging activity. Six different methanolic extracts (the peel, flesh and seed of Eleiodoxa conferta at young and mature stages) were studied by using Folin-Ciocalteu method and DPPH radical scavenging assay. Yellow colour bands were developed by all fruit parts Eleiodoxa conferta on the chromatogram, indicated the presence of antioxidant of all the methanolic extracts. In Folin-Ciocalteu assay, young flesh extract has the highest total phenolic content at 74.71 ± 0.00 ͨ GAE mg/5g sample. For DPPH radical scavenging assay, the young flesh also has the lowest IC₅₀ at 0.08 ± 0.00 mg/mL while the BHT standard was 0.01 ± 0.00 mg/mL. There are significant positive correlation (R² = 0.857) of total phenolic content and free radical scavenging activity of sample concentration at 5 mg/mL. As a conclusion, the research suggest that all the parts of Eleiodoxa conferta fruit have antioxidant activity, the young flesh has the highest antioxidant activity significantly (p<0.05). The fruit has the potential to be exploited as natural antioxidant.