Callus induction from leaves of polygonum minus (kesum) - an important medicinal plant

Polygonum minus has been reported to contain valuable metabolites production in P. minus. The study was conducted to induce the callus from the leaves of P. minus and to study the effect of different type of hormone used on callus induction of P.minus. The sterilization method was used 0.1% of mercury chloride for 5 minutes. Naphthalene acetic acid (NAA) and 33- naphthoxy acetic acid (NOA) concentrations in the range of 1.0, 2.0, 3.0, 4.0 and 5.0 mg/L were used in a matrix of combinations with benzylaminopurine (BAP) concentrations in the range of 0, 0.5 and 1.0 mg/L as plant growth regulators (PGRs) to induce callus cultures. Media that were supplemented with 3.0mg/L NAA and 0.5mg/L BAP , 3.0mg/L NAA and 1.0mg/L BAP, 1.0mg/L NOA and 1.0mg/L BAP, and 2.0mg/L NOA and 0.5mg/L BAP were effective for callus induction (100 % of the explants produced callus) after 4 weeks the whitish and friable callus were produced. For further research and conservation of all species of plants including medicinal plants needed to preserve natural remedies nature. Advances in plant tissue culture will allow rapid multiplication and sustainable use of medicinal plants for future generations.