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Effect of nutritional factors on protease production by isolate pjk 2 2 (1) 2


Chiu, Li Mei (2014) Effect of nutritional factors on protease production by isolate pjk 2 2 (1) 2. Undergraduate Final Project Report thesis, Faculty Of Agro - Based Industry. (Submitted)


Study on the effect of nutritional factors and optimization for maximum protease production was carried out using the isolated bacteria strain PJK 2 2 (1) 2 which collected from Jeli Hot Spring (Kelantan, Malaysia). The bacterium produced protease at maximum rate after 24 hours of incubation at 50°C with agitation speed of 150 rpm and 4% (v/v) starter culture. The best carbon, organic nitrogen and metal ion sources were glucose, tryptone and calcium chloride with protease activity 39.11 U/ml, 39.67 U/ml and 40.78 U/ml respectively. Meanwhile, the addition of inorganic nitrogen source and amino acid reduced the protease production. The combination of glucose, tryptone and calcium chloride produced the maximum protease production was 40.78 U/ml.

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Additional Metadata

Item Type: Undergraduate Final Project Report
Collection Type: Final Year Project
Date: 2014
Call Number: SBT 14008
Supervisor: Dr Noor Azlina Ibrahim
Programme: Bio - industrial Technology
Institution: Faculty Of Agro - Based Industry
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