The water qualities of man-made lakes in central region of peninsular Malaysia are very vulnerable to the external change e.g human disturbance and natural factors such as rainfall. This study focuses on physic-chemical assessment of lake water quality of Kepong Botanical Garden (KBG) Lake in FRIM, Kepong, Selangor and to compare the obtained result with lake water quality of Varsity Lake, University of Malaya, Wilayah Persekutuan Kuala Lumpur and Putrajaya Lake, Wilayah Persekutuan Putrajaya. A total of eleven sampling stations were established at KBG Lake; seven of sampling stations are inlet stations and four stations were at the middle of KBG Lake. Thirteen water quality parameters were analyzed based on in-situ and ex-situ analysis for a week sampling period and laboratory analysis were done according to HACH and APHA methods. Based on Interim national Water Quality Standard, Malaysia (INWQS) classification, sulphate (SO4), nitrate (NO3), conductivity, temperature, total dissolved solid (TDS), ammonium nitrogen, and turbidity were under class I category, while Dissolved oxygen (DO) and Total suspended solid (TSS) were classified as class II and pH under class III. Moreover, water quality in KBG Lake shows concentration effect trend to total rainfall per day along the one week where the most of parameters (DO, pH, turbidity, TSS, SO4, NO3, PO4, salinity, Conductivity, temperature, ammonium nitrogen) affected except quantum yield and TDS. The healthiest lake water obtained was at Putrajaya Lake, followed by KBG Lake and then Varsity Lake. A sustainable management of man-made lake in central region of Malaysia is the key factor for a clean lake water quality.