Occurrence of antibiotic resistance of salmonella sp. isolated from snakeskin gourami

The aim of the present study was to test the antibiotic susceptibility of Salmonella isolates from snakeskin gourami against 17 antibiotics and to reveal the exposure level of the antibiotics known as Multiple Antibiotic Resistance (MAR) index. Salmonella were isolated using Xylose Lysine Deoxycholate (XLD) medium. A total of ten bacterial isolates were randomly selected for antimicrobial sensitivity test using Kirby-Bauer disk diffusion method onto Tryptone Soy Agar (TSA) media. Seventeen antibiotics, namely fosfomycin, colistin sulphate, chloramphenicol, spiramycin, ampicillin, erythromycin, novobiocin, doxycycline, sulfamethoxazole, compound sulphonamides, florfenicol, lincomycin, flumequine, nalidixic acid, gentamicin, tetracycline and amoxicillin were tested. The result of the study indicated that Salmonella isolates sensitive to antibiotic tested which are gentamicin, florfenicol and nalidixic acid with their percentage values of 100%, 90% and 80% respectively whereas Salmonella isolates was found 100% resistant to ampicillin, lincomycin and amoxicillin. High level of multiple antibiotic resistances index (0.48) was observed within the Salmonella isolates. This indicated that Salmonella bacteria from snakeskin gourami was highly susceptible to some antibiotics which may cause significant impact on aquaculture farming practices particularly in disease management.