Snakeskin gourami (Trichogaster pectoralis) is favoured significantly for food and as an ornamental pet fish due to their esthetical value and certain health benefits assembles fish and fishery products worth worldwide. Rearing aquaculture fish species is a growing interest nowadays especially for hobbyists but will eventually lead to the possibility of disease and bacterial infection between them. Aeromonas spp. is the most notable bacteria that gravely affects Gourami species perhaps human being. In this research, Aeromonas hydrophila was being used to evaluate its virulence through isolation using Kirby-Bauer disc diffusion method against 16 types of antibiotics selected: colistin sulphate (25 μg/disc), chloramphenicol (30 μg/disc), compound sulphonamides (300 μg/disc), doxycycline (30 μg/disc), lincomycin (15 μg/disc), nalidixic acid (30 μg/disc), novobiocin (30 μg/disc), oleandomycin (15 μg/disc), oxolinic acid (2 μg/disc), spiramycin (100 μg/disc), sulphamethoxazole (25 μg/disc), tetracycline (30 μg/disc), flumequine (30 μg/disc), erythromycin (15 μg/disc), fosfomycin (50 μgdisc) and florfenicol (30 μg/disc). In addition, the bacterial isolates were randomly chosen for further diagnosed under pathogenicity test. Resistance cases to the tested antibiotics have been described. The results showed that 68.75% case of antibiotics sensitive recorded among 10 bacterial isolates and this followed by resistance case that 27,50%, while for intermediate case were 3.75%. Aside from that Multiple Antibiotics Resistance(MAR) index was measured with value 0.275 showed that Snakeskin gourami has exposed to the tested antibiotics which was very high-risk.