The potential of antibiotics susceptibility testing to pseudomonas spp. isolated from climbing perch, anabas testudineus raised in Umk

The aim of the present study was to examine the potential of antibiotics susceptibility testing to Pseudomonas spp. isolated from climbing perch fish, Anabas testudineus raised in UMK. Pseudomonas spp. were recognized as a pathogenic bacterial in worldwide aquaculture. Therefore, this study was carried out to collect the information about the potential use of multiple antibiotics to the selected climbing perch fish against the Pseudomonas spp. and to study the effectiveness antibiotic to control Pseudomonas spp. in Anabas testudineus. In the present study, Pseudomonas spp. was isolated using Glutamate Starch Pseudomonas (GSP) medium. The antibiotic susceptibility testing was performed by disc diffusion method on Tryptone Soy Agar (TSA), using twelve types antibiotic to a total of ten isolates of Pseudomonas species. Twelve antibiotics, namely doxycycline (30 pg), nalidixic acid (30 pg), novobiocin (30 lig), oxolinic acid (2 lag), ampicillin (10 pg), amoxicillin (25 pg), chloramphenicol (30 pg), sulphamethoxazole (25 pg), flumequine (30 pg), spiramycin (100 pg), tetracycline (30 i_tg) and oleandomycin (15 pg) were tested in the present study. The results of the present study indicating that all isolates were sensitive to doxycycline, nalidixic acid, oxolinic acid, chloramphenicol, sulphamethoxazole, flumequine and tetracycline (100%). On the other hand, present bacterial isolates (100%) were found to be resistant to ampicillin and amoxicillin while 7/10 (70%) were resistant to oleandomycin and novobiocin. Most of the bacterial isolates 5/10 (50%) were intermediary sensitive to spiramycin whereas 1/10 (10%) bacterial isolates were resistant to spiramycin. Multiple antibiotic resistances (MAR) index of 0.29 observed in this study, indicates that Pseudomonas spp. isolated from climbing perch fish raised in UMK may have received high exposure to the tested antibiotics.