Comparison between embryo culture technique and the effect of acid and heat treatment of the germination of wild musa acuminata ssp.malaccensis

The aim of the present study was to examine the effect of acid and hot water treatment on the wild banana seed germination rate and to identify the faster technique to increase the germination in vitro percent. Seed were treated using three techniques which is Nursery, Seed culture and Embryo Culture. In nursery involved four different of treatment that is scarification with 10% HCI and 10% H2SO4 for 15 min; hot water treatment for 40°C and 60°C for 14 hours and 8 hours respectively. For the Seed Culture by in vitro technique involved six different of treatment that is scarification with 10% HCI, 15% HCI, 10% H2SO4, 15% H2SO4 for 15 min; hot water treatment for 40°C and 60°C for 14 hours and 8 hours respectively. By in vitro embryo culture technique involved two different of technique that is embryo culture halves cutting and Zygotic embryo culture. The percentages of germination in H2SO4 treated seeds were higher (20%) than in HCI treated seeds (18%). High germination of zygotic embryo rescue technique had been observed with the percentage of 28.86% and followed by the embryo recue halves cutting technique about 28.67%. The zygotic embryo rescue technique is the best way to increase the germination rate wild banana seeds compared to in vivo technique.