Isolation of bacteria from soil surrounding of rafflesla Kerri Meijer in Lojing Highlands, Kelantan

Upstream Lojing Highland, Kelantan is chosen as the study area to isolate the bacteria from soil surrounding the Rafflesia kerri flower. Rqf/lesia kerri is attraction for tourist in the Lojing Highlands. The objectives of this study is to assess the distances association of soil bacteria from Rqfflesia kerri by identify the morphology characteristics of soil bacteria using Gram staining method. Soil samples were collected with different distances from the Rafflesia flower which are 20, 40 and 60 meters. A total number of 34 isolated bacteria were obtained growth on the Nutrient Agar plate. Gram staining method was used to determine Gram positive and negative bacteria by observed under light microscope. Morphology characteristics bacteria were characterized through form and Gram staining. The percentage form of bacteria obtained are as follows: irregular (38%), filamentous (29%), spindle and rhizoid both (12%) and circular (9%). Irregular form is the common shape for soil bacteria. Result from the Gram staining shows 65% of bacteria are from the Gram positive bacteria, meanwhile Gram negative are 35%. The results also identified that five species of bacteria inhabited on soil surrounding of Rqf/lesia kerri flower as follows: Corynebaclerium diphrheriae. Bacillus sublilis. Staphylococcus aureus, Escherichia coli and Spirilium volurans. As conclusion, number of colonies of soil sample collected from distances near to Rafllesia kerri ‘flower higher than distances far to flower and mostly the soil bacteria collected is irregular form and positive Gram bacteria.